Peptide-based thermal unfolding curves (PBTUC) and browser application (07.01.2017)
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Citation: 
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Leuenberger et al., 2017 [full citation to be added], "Peptide-based thermal 
unfolding curves (PBTUC) and browser application ", doi:10.7910/DVN/ZT1EZG, 
Harvard Dataverse

Description:
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This archive contains high-quality peptide-based unfolding curves 
as .png files for the E. coli, S. cerevisiae, T. thermophilus 
and human proteomes. The archive can be browsed manually or using 
the attached R application (shiny based). 
 
The curves show peptide intensities along a temperature gradient from 37 to 76
degrees C (for the E. coli, S. cerevisiae or human proteomes) or from 55 to 93 
degrees C (for the T. thermophilus proteome). 

Png file names and curve titles are according to the following nomenclature:

“protein name”_”peptide sequence”_”modifications”, 

where modifications include:  
“amino acid number_C”, carbamidomethylated cysteine residue
“amino acid number_M”, oxidized methionine residue  

Protein names are according to Uniprot accession numbers.

Usage of the PBTUC Browser:
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Requirements:

- R version 3.2.3 or later
- Shiny version 0.13.2 or later
- Contents of the dataverse repository: all scripts and all plots should be saved in one folder and all files should be unzipped. 

Launch:

The PBTUC browser can be launched from the command line (Windows users, please
make sure to be in the directory of R when launching the PBTUC browser) 
or terminal (Mac/Linux) using the following command line:

Rscript run.R

E.g. on a Mac: Rscript run.R 
E.g. on a Windows machine: C:\Program Files\R\R-3.2.3\Rscript run.R 

A browser page will open and you will be able to browse the data based on the Uniprot protein accession number. 
 
